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Description
Cells were resuspended in ice-cold PBS plus 0.1% BSA and processed using a BD LSR II flow cytometer (10,000 cells/sample) for receptor trafficking, or BD LSRFortessa X-20 for surface downregulation
doi:10.15537/smj.2021.42.5.20200676 7

Patients exercised daily and ate a reduced-calorie diet

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