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295 milliosmoles/L) containing (in mM): 138 NaCl, 5.6 KCl, 2.6 CaCl 2 , 1.2 MgCl 2 , 10 HEPES (adjusted to pH 7.4 with NaOH), and supplemented with 11.1 mM glucose, 20 l ml 1 FBS, 1 l ml 1 Pluronic F-127, and 1 M fura-2 acetoxymethyl ester (fura 2-AM