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For cryo-confocal data acquisition, the cryo-holder was transferred onto the cryo-Linkam stage (Linkam Scientific) and mounted onto a ZEISS Axio Imager.Z2 fitted with a LSM900 confocal scan head equipped with 3 confocal channels, including the Airyscan 2 detector for super resolution

That gap, about 3 to 4 percentage points, is statistically meaningful but requires context to interpret properly

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