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It is influenced by many factors, including excessive energy intake and high androgen levels that promote a change in body fat distribution with an increase in visceral fat volume [9,36]

In order to avoid further HIF-1 degradation during whole cell lysates preparation, cells were lysed directly with NuPAGE LDS Sample Buffer (1X) (Thermo Fisher Scientific, San Jose, CA, USA) in the presence of protease and phosphatase inhibitors cocktail (Cell Signaling Technology, Danvers, MA, USA), similarly to ref