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Description
The primer sequences were the following: sense: 5-ACCGCCCTGAGATTACTTTTCTG-3, antisense: 5-AGTTCTCTTTCCAGGTTCACCAC-3 and probe: 5-6-FAM-CGCAGTCACGCAACCTGGATTACAA-ZEN-3 (Iowa Black FQ)
Further reciprocal mutagenesis studies will be required to identify key interactions responsible for the contrasting effects of exendin-phe1, exendin-asp3 and other analogs, facilitating future design of agonists with even greater selectivity
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Fernandez ML, West KL
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