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In addition to this open 7TMD pocket, the GCGR structure revealed two other distinct structural features that are not observed in the CRF 1 R structure, namely, a long N-terminal extension of TM helix 1 (defined as stalk region) and a long helix 8 at the C-terminal end of the receptor that is differently oriented than helix 8 observed in the crystal structures of other GPCRs (Hollenstein et al., 2014)

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115 Compared with the structure of human GLP-1(7-37), the amino acid sequence of semaglutide contains diaminoisobutyric acid at position 8, arginine at position 34, and acylated lysine at position 26