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Supplementary Information Acknowledgements The authors thank Edson Fernandes de Assis for performing the cytokine assays with the multiplex platform and the Fiocruz network of technological platforms and the confocal platform from the Oswaldo Cruz Foundation, Fiocruz, Rio de JaneiroManguinhos Mar campus

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These findings highlight the complexity of metformins effects, which may be age-, genotype-, and disease-context dependent, reinforcing the need for further experimental and clinical validation to clarify its therapeutic potential and risks in AD prevention across different populations