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Follow-up data from extended analyses confirmed that weight regain after stopping liraglutide follows a similar trajectory to other GLP-1 medications, though the absolute numbers are smaller because less weight was lost during treatment

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Islets were then lysed using acidic ethanol (75% ethanol, 15 mM HCl), sonicated 3X 10 seconds and centrifuged at 1,000 rpm for 3 minutes, and supernatants collected to assess internal insulin content and stored at -20C