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The cells were incubated in a basal medium consisting of serum-free DMEM/F12 medium, supplemented with 17 M D-pantothenic acid (Sigma), 33 M d-biotin (Sigma), 5 g/ml transferrin (Sigma), 1 nM triiodothyronine (Sigma), 5 g/ml insulin (Sigma), 500 g/ml fetuin (Sigma), and 5 ng/ml selenium (Sigma)

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Many people find they feel more nauseous than usual when drinking, even with smaller amounts than they previously tolerated

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Conditions or therapies that predispose to acidosis (i.e., renal disease, severe respiratory disorders, status epilepticus, diarrhea, surgery, or ketogenic diet) may be additive to the bicarbonate lowering effects of QSYMIA
