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Acetonitrile (125 ml) was added to the obtained compound at 25-30C

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Several observations suggest a possible action of GLP-1 on thirst-regulatory mechanisms, since GLP-1R mRNA has been located in brain areas related to the control of thirst, such as the preoptic area, glial cells lining the third ventricle and, especially, the neurons of the PVN, which is a key station for water balance regulation through the antidiuretic effects of vasopressin released by its projection to the neurohypophysis [44]