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After that, cells expressing GIPR and GLP-1R were incubated with 1:300 anti-Flag primary antibody (diluted with PBS containing 5% BSA, Sigma), and those expressing GCGR were incubated with 1:50 anti-GCGR antibody (diluted with PBS containing 5% BSA, Abcam) at RT for 1 h

Step 7: Initiate Therapy with Structured Monitoring Begin GLP-1 therapy with a clear, structured plan for monitoring progress and adjusting treatment as needed

The semi-structured nature of the interviews allowed for open-ended questions to be asked, designed to elicit discussion, which were guided by an interview schedule, to ensure the interviews were systematic and comprehensive [28]

Data are presented as means SDs, and statistical significance was determined by two-tailed unpaired Students t tests with GraphPad Prism software 8.3.0

Tagrisso is specifically indicated for people with exon 19 deletions or exon 21 mutations