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The recording electrodes had a resistance of 3 to 5 M when filled with pipette solutions containing 120 mM K-gluconate, 10 mM KCl, 10 mM 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES), 1 mM CaCl 2 , 1 mM MgCl 2 , 5 mM ethylene glycol-bis(2-aminoethyl ether)-N,N,N,N-tetraacetic acid (EGTA), 2 mM Mg-ATP, and 0.03 mM Alexa Fluor 488 hydrazide dye