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10 , 774785 (2022) F.A

T., & Bird, A

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Determination of protein concentration in supernatant using BCA protein Assay Kit (Applygen Technologies, Beijing, China), and equal amounts of total protein underwent 10% SDS-PAGE and were transferred to polyvinylidene difluoride membranes, which were blocked with 5% non-fat milk, and incubated with different primary antibodies overnight at 4 C