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Cells or islets were incubated for 24 hours post-transfection / transduction before labelling with 1 M SNAP-Surface fluorescent probes (New England Biolabs) for 15-20 minutes for cells, or 7 minutes for islets, in full media at 37C

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Nevertheless, studies in human subjects with type 2 diabetes surprisingly showed well-preserved insulinotropic activity of both GLP-1 [736 amide] [5] and GLP-1 [736] that was accompanied by a short-term reduction in plasma glucose in the normal fasting range in patients previously characterized by persistent hyperglycemia [6,7]

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The SELECT trial showed a 20% reduction in cardiovascular events among overweight or obese patients taking semaglutide