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For studies with a calorie-restricted and body-weight-loss-matched control group, HFHS diet (58 kcal% fat, D12331i, Research Diets) was weighed and provided as one separate pellet per mouse at the time of injection

afterwards, DR tends to improve as the body establishes a new homeostasis

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Effects on glucose metabolism Increased insulin secretion in a glucose-dependent manner Decreased glucagon secretion Increased insulin sensitivity Possible decrease in blood glucose due to the above-mentioned effects

Nevertheless, recent advances in understanding the molecular mechanisms, regulatory factors of GLP-1 secretion, signaling pathways, and transcription factors that are effective in endocrine cell differentiation may pave the way for the development of effective applicable medications that can increase GLP-1 secretion and improve L-cell differentiation