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The mice were then decapitated and the entire brain was removed and immediately submerged in ice-cold, carbogen-saturated (95% O 2 and 5% CO 2 ) aCSF (126 mM NaCl, 2.8 mM KCl, 1.2 mM MgCl 2 , 2.5 mM CaCl 2 , 1.25 mM NaH 2 PO 4 , 26 mM NaHCO 3 and 5 mM glucose)

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Why Duration and Indication Matter in Treatment Selection Phentermine's approval for short-term use reflects decades of evidence focused on acute appetite suppression

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We identified six biophysical properties that can be predicted from a sequence using the ProtParam module ( from the biopython Python package 45 : (1) the isoelectric point in neutral pH, (2) GRAVY (grand average of hydropathy) 46 , (3) the instability index 47 , (4) aromaticity, (5) the molar extinction coefficient and (6) molecular weight