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Lysates were sonicated 3X 10 seconds, centrifuged at 10,000 rpm for 10 minutes and incubated with anti-FLAG M2 affinity gel (A2220, Sigma Aldrich) rotating overnight at 4C to immunoprecipitate the SNAP/FLAG-hGLP-1R
[DOI] [PubMed] [Google Scholar] 258.Goeddel, D
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02Side-effect tolerability Lower dosing typically means a quieter side-effect profile, which makes long-term adherence realistic for the patients who need it most
