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Cells were lysed using 100 l of cell lysis buffer containing a protease inhibitor cocktail (Roche) followed by centrifugation at 13,000 g at 4C for 10 min
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Studies show that LH rises within minutes of administration, and testosterone levels increase within 12 hours
To this end, patients were required to either have coverage (Optum) or have prior visits (MGB) at least two years prior to their initial PD diagnosis
