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Furthermore, in another study [47, 60], one semaglutide analogue demonstrated a binding affinity to GLP-1R that is more than two orders of magnitude (113.3 times) higher than native semaglutide, achieving a K d of 3.0 10 8 M compared to the K d of 3.4 10 6 M for native semaglutide

J Sex Med (2011) 8:27283

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The substitution (Y10 P in tirzepatide) and modification (lipidated K10 P in peptide 20) stabilized the binding of dual and triple agonists by newly-formed interactions with residues surrounding the TM1-TM2 cleft, a phenomenon unseen in the case of GLP-1 27

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