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The small intestine was divided into duodenum, proximal jejunum, distal jejunum, and ileum (1:1:1:1 ratio) and flushed with ice cold phosphate-buffered saline (PBS)

To ensure safety and efficacy, never use semaglutide that shows visual changes, has been exposed to extreme temperatures, or is beyond the labeled expiration date

Small shifts in storage conditions, thaw-refreeze practices, or internal standard selection can accumulate into significant variability, making it difficult to interpret subtle but biologically meaningful differences in endpoints such as glucose tolerance, body-composition proxies, or signaling-pathway activation