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We conducted a literature review about this topic because I see many patients prescribed GLP-1 receptor agonists with erectile dysfunction and also on testosterone replacement therapy

Retatrutide adds glucagon receptor agonism to the GLP-1 and GIP targets already covered by tirzepatide, which is hypothesised to further stimulate hepatic fatty acid oxidation and lipid clearance

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Proteins were then transferred onto a polyvinylidene difluoride (PVDF) membrane using an iBlot2 PVDF stack (Invitrogen) and blocked with 5% bovine serum albumin in tris-buffered saline with 0.1% Tween-20 (TBST) for 1 hour at room temperature