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Guidelines for cardiovascular risk reduction in patients with type 2 diabetes: JACC guideline comparison
[DOI] [PubMed] [Google Scholar] 158.Coupe B, Ishii Y, Dietrich MO, Komatsu M, Horvath TL, Bouret SG
Endocrine 50 , 620626 (2015)
After purification, islets were cultured overnight at 37 C in a humidified atmosphere of 95% air and 5% CO 2 in RPMI 1640 medium supplemented with 10% FBS, 100 units/ml penicillin and 0.1 mg/ml streptomycin
8-12 weeks is optimal for ramp, and daily use is critical
