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15 Months of Progress After 15 months of consistent treatment with Tirzepatide at a dose of 7.5 mg/week, JHs results were nothing short of inspiring: Follow-Up Bioimpedance Spectroscopy Measurements: Weight: 162 lbs (a 78-lb loss) Body Fat Percentage: 32.9% (down 12.8%) BMI: 23.3 (a reduction of 10.1 kg/m) Body Fat Mass: 53.2 lbs (a decrease of 56 lbs) Lean Body Mass: 109 lbs (down 21 lbs) Skeletal Muscle Mass: 59 lbs (loss of only 12 lbsjust 15% of her total weight loss, less than the typical 20%) ECW/TBW: Slight improvement in the right leg to 0.396

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-arrestin 2 recruitment HEK293T cells (3.2 10 6 cells/10 cm plate) were grown for 24 h before transiently transfection with 15 g plasmid containing GIPR, GLP-1R or GCGR tagged with Rluc8 and -arrestin 2 with a Venus-tag at the N terminus at a ratio of 1:9 by Lipofectamine 3000 transfection reagent
39,40,43 There may be several possible explanations for the failure of GLP-1 RAs in HFrEF studies
