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For immunofluorescent staining, tissue sections were incubated with anti-insulin antibody (Abcam), followed by a secondary fluorophore-conjugated antibodies (Invitrogen), or with FITC conjugated anti-mouse CD11c (BD Bioscience), or with Cy5 conjugated anti-mouse F4/80 (eBioscience) and counterstained with 4,6-diamidino-2-phenylindole (DAPI) (Sigma)

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However, recent FDA decisions have removed these medications from the list of substances eligible for compounding, meaning that compounded semaglutide and compounded tirzepatide are no longer generally available

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Regarding the inclusion of preprints, its inclusion was carefully justified to reflect cutting-edge developments, and we emphasize that its preliminary nature does not detract from the overall evidence base