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Pang et al

We extracted the culture medium after cell culture for ELISA detection

Patatin-like phospholipase domain 2 (PNPLA2) is also involved in lipid homeostasis [54, 55]

After three brief washes with PBS, the samples were incubated with 10 mM MgCl 2 in PBS for a further 5 minutes and then mounted on a well slide in freshly prepared GLOX buffer (100 mM Tris-HCl pH 8, 10% w/v glucose, 0.4 mg/mL glucose oxidase, 64 g/mL catalase) supplemented with 10 mM MEA and sealed with two part silicon (eco-sil speed, Picodent)

2024), ultimately leading to decreased adiposity and body weight (Drucker 2022), decreasing hepatic steatosis and circulating lipid levels (Targher et al