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In hot weather, the injection site may be sweaty, which interferes with alcohol swab cleaning and can increase infection risk if not managed

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Plasma SCFA Plasma was extracted and analyzed using ultra-high-performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS) as previously described in detail

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34 Diagnostic approaches Clinical diagnosis Although consensus clinical diagnostic criteria have been published 35 and validated, they were published before comprehensive laboratory testing for PWS was available