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After blocking with 5% BSA, the sections were incubated with 1:200 anti-GLP-1 (ab23468, Abcam), followed by detection with secondary antibodies Alexa Fluor 488 conjugated goat antibody to mouse IgG (A-11029, ThermoFisher

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Tirzepatide, however, works through a dual mechanism by activating both GLP-1 and GIP receptors