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Samples then were eluted with elution buffer [1% (w/v) SDS, 250 mM NaCl, 1 mM EDTA, 10 mM TRIS pH 8] for 10 minutes at 65C, and analyzed using SDS-PAGE on an 8% or 12% acrylamide gel

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Treatment of patients with type 2 diabetes mellitus with doses of Vildagliptin 50 - 100 mg daily resulted in significant improvements in beta-cell function

Additionally, there was an average improvement in glycated hemoglobin levels of 0.8 point ( 0.6), highlighting the positive impact of the therapy