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Cells were cultured in Dulbeccos modified Eagles medium (DMEM) (Gibco, NY, USA) supplemented with 10 % foetal bovine serum (FBS) (Gibco, NY, USA) and were maintained in a humidified incubator at 37 C and 5 % CO 2

Samples were processed on ice and then were centrifuged at 13,200 g for 10 min at 4 C and 1X protease inhibitor in a microcentrifuge

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Perhaps the better approach is to use an algorithm that includes gastric ultrasound, rapid sequence induction if GA is required, and greater use of clinical signs

Hypoglycemia, though uncommon with tirzepatide monotherapy, can occur in individuals taking concurrent diabetes medications, particularly insulin or sulfonylureas