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Mobile phases consisted of Solvent A (60% ACN, 40% HO, 10 mM Ammonium Formate with 0.1% Formic Acid) and Solvent B (90% IPA, 10% ACN, 10 mM Ammonium Formate with 0.1% Formic Acid)

The turbulence causes mechanical degradation of peptide bonds

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