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The complex was then concentrated using an Amicon Ultra Centrifugal Filter (MWCO 100 kDa) and subjected to size-exclusion chromatography on a Superdex 200 Increase 10/300 column (GE Healthcare) that was pre-equilibrated with 20 mM HEPES pH 7.4, 100 mM NaCl, 2 mM MgCl 2 , 1 M exendin-4 or 10 M oxyntomodulin, 0.01% (w/v) LMNG and 0.0006% (w/v) CHS to separate complex from contaminants

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In -cells, TMACs catalyze conversion of ATP to cytosolic cAMP, a second messenger that activates either protein kinase A (PKA) or the cAMP-regulated guanine nucleotide exchange factor designated as Epac2

Hinzu kommen die Vorteile im Hinblick auf die Vermeidung von Hypoglykmien und eine Chance auf eine Gewichtsreduktion (33)

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