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Thirty-five minutes (stabilization period) after completion of the surgery, the pylorus was clamped, and the rats received intragastric or intraduodenal load of glucose (2 g/kg BW) or PBS in a volume that did not distend the stomach

Blood glucose experiment in healthy mice After the acclimation period (at least 5 days), mice (male C57BL/6J mice (Charles River laboratories) 8 weeks old, 2025 g) were randomized into 10 groups ( n = 3 per group) according to their body weight

Pinch the area up between two fingers to form a fold to inject into, pinching skin and fat tissue, avoid bunching up any muscle with it

Tissues were embedded and sectioned at a thickness of 12 m using a cryostat