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Several transgenes were generated and maintained on RNAi feeding bacteria

The protein sample was mixed with lipid (monoolein/cholesterol 10:1 by mass) at weight ratio of 2:3 using a syringe mixer

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Intriguingly, a blockade of central estrogen receptors reduces the effect of a GLP-1R analog (exendin-4) on food-motivated behavior in both sexes, indicating that estrogen may modulate the effects of GLP-1 analogs not only in females but also in males (40)
