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The alendronate group demonstrated significant improvement in morphological features of bony samples with obvious records of apparent intact trabecular bone with up to a 2.4-fold increase of relative trabecular bony area

Samples were fixed with 4% PFA at room temperature for 30 min, permeabilized with 0.1% Triton-100 at room temperature for 15 min, and blocked with 5% bovine serum albumin (BSA) solution at 37 C for 1 h

GLP-1 is also secreted centrally from the brain by the release of preproglucagon neurons

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