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Mitochondrial isolation Mitochondria were isolated from HT1080 cells lines using a Mitochondria Isolation Kit for Cultured Cells (Thermo Scientific, #89874) as per the manufacturers instructions

In all, 1 l of lysates were used as templates for a genotyping PCR using Q5 polymerase (NEB, #0491)

The resulting product ions were compared to product ion spectra of standards in the LIPID MAPS database (Nature Lipidomics Gateway, www.lipidmaps.org) and/or previously published data 75,76

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