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Urinary proteins were fractionated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis as described previously ( iTRAQ Labeling and 2D-HPLC-MS/MS Analysis Each 200 g extract sample was prepared using standard digestion, reduction, and alkylation procedures prior to isobaric tag for relative and absolute quantitation (iTRAQ) labeling ( 18 2.1 50 mm, 1.7 m)

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As a result, positive outcomes in rodent studies may partly reflect an upregulation of endogenous antioxidant defenses rather than the effects of the administered antioxidant itself

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