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Description
2.2 Nile Red vital staining Nile Red (Sigma-Aldrich) was added in DMSO to a final concentration of 5 mM as stock solution
A nutritional deficiency occurs when the body does not absorb or get enough nutrients from food
This die stains neutral lipids (triglycerides, mono and diglycerides, cholesterol esters, etc.) to provide a semiquantitative measure of total neutral lipids
For BT549 CRISPR Knock-in experiment, lentiCas9-Blast (Addgene plasmid #52962) and lentiGuide-Puro (Addgene plasmid # 52963), both gifts from Fenz Zhang, were used
Developmental cell, 2019
