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There is enough primary data to say AOD-9604 does something to fat metabolism in rodents the lipolytic and fat-oxidation readouts are real and reproduced across more than one study

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The cells were fixed in 4% paraformaldehyde for 15 min, followed by permeabilization using 0.3% Triton X-100 (Beyotime, China) for 15 min, washed with phosphate-buffered saline (PBS) and blocked with 10% bovine serum albumin (Beyotime, China) for 1 h