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Store lyophilized material at 4 F (20 C) or below Allow vial to reach room temperature before opening Protect from light, heat, and humidity Use sterile laboratory equipment during preparation Avoid repeated freezethaw cycles Label reconstituted samples with preparation date and concentration Following these practices helps support consistent and reproducible laboratory results

Our data suggest that choline can promote liver health by maintaining cholesterol homeostasis.10 Choline induces glucose and insulin intolerance in Pemt(-/-) mice through modulating plasma glucagon and its action in liver.11 Choline supplementation decreased the concentration of liver triacylglycerol during the first 4 wk after parturition