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Next, 10 g/mL (100 L/well) of His-tagged human FcRn, diluted in PBS/T/M (pH 5.5 or pH 7.4), was added for 1 h at room temperature, followed by washing as before

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In gluten-free diet, gluten-free foods (such as vegetables, fruits, legumes, meat, eggs, and fish) are preferred instead of gluten-containing grains [28, 29]

Despite their utility, a significant challenge in iHLO research lies in determining the optimal developmental timepoints for liver-like functionality, which is critical for their effective use in pharmacological and toxicological studies

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