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Protein samples (20 g) were separated on NuPage Novex 4%12% Bis-Tris gels (Life Technologies, Carlsbad, CA, USA), then transferred to PVDF membranes (Rockford, IL, USA), which were blocked with non-fat milk and incubated with primary antibodies (OXPHOS complexes
Moderate alcohol consumption, defined as up to one drink per day for women and up to two drinks per day for men, is generally considered acceptable for most patients, though individual tolerance and circumstances should be considered

Di Marzio L, Moretti S, D'Alo S, et al

Acute improvement of cardiac function with intravenous L-propionylcarnitine in humans