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Step 3: Check for Blood Vessels (Aspiration) This next step, known as aspiration, is a quick but critical safety check

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FDAs 2023 Category 2 placement cited significant immunogenicity risk for some routes of administration, plus impurity and API characterization concerns

Gastroenterol 135(5):14421447

A faint pale-yellow color can occur with small-molecule compounds in aqueous solution, but a deepening yellow shade often indicates oxidation or degradation from light or temperature excursions