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The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH 2 O and PBS, and then probed with the primary antibody (HA601200) at 1/1,000 dilution for 1 hour at room temperature

Overall, there were no differences in ECAR in any condition or in response to injection of glucose (G), oligomycin (O), or 2 deoxy-D-glucose (2DG) (see the cartoon in Fig

Tissue culture raised mountain ginseng adventitious root (TCMGARs) extracts at dosage levels of 250, and 500 mg/kg significantly reduced the blood glucose, total cholesterol, and triglyceride content in STZ-induced diabetic rats [38]
In addition, redox conditions vary between cell-based/organoid cultures and in vivo conditions, which is illustrated by the frequent requirement for system x c - in cell culture, whereas system x c - knockout mice are viable (McCullagh and Featherstone, 2014)
