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Stanhope R, Albanese A, Hindmarsh P, et al
The differences at the glutathione binding site and in particular a deformation caused by a Leu-Ile exchange indicate why the E
doi: 10.1016/j.fct.2018.10.054
Plant Stress 2, 100040
His-tags have lower binding specificity compared to strep-, FLAG-, and GST-tags, which is the major limitation of nickel affinity chromatography (Figure 5) (Kimple et al, 2015)
