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To make the mutated binding site construct, four bases of the putative miR18a binding site on Gclc 3 UTR were mutated using sitedirected mutagenesis, as per manufacturer's instructions (QuikChange Lightning SiteDirected Mutagenesis Kit, Agilent)
There is no solid proof that B12 shots cause weight loss in people who are not deficient
doi: 10.1038/s41586-022-05593-1 12 ConfortiFPalaLDi MitriDCataniaCCocorocchioELaszloDet al
P., Tobbia, I
