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Rho/Rho-associated coiled-coil forming kinase pathway as therapeutic targets for statins in atherosclerosis

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Ensuite, une injection de 1000mcg peut tre donne une fois par mois

2.2 Laboratory-scale bioreactor cell culture At the laboratory scale, MRC-5 cells were cultured in 3 L glass bioreactors with a BioFlo 320 controller (Cat#: 1379962911, Eppendorf, Hamburg, Germany) on 1 g/L Cytodex 1 microcarriers (Cat#: 17548702, Cytiva, Marlborough, MA) or in iCELLis Nano fixed-bed bioreactors (Pall Corporation, Port Washington, NY) with a 10 cm bed height with WMEM and 10% BCS controlled at a target temperature of 37C and pH of 7.2 as described previously (Swartz et al., 2022)

Check that the very end of the needle is fully submerged in the insulin (not in air) to avoid air draw-up