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Analysis was performed using Oroboros Datlab 7 ( and further normalization was done to correct for day-to-day variations by normalizing to the average maximal respiration of the sham group for each day

10.7554/eLife.70349 17

GST activity measurements GST activity in crude enzyme extracts from Arabidopsis roots was determined using a commercial GSH transferase assay kit (Sigma-Aldrich)