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The MDH2 gene is located on chromosome 7q11.23 and is composed of 10 exons that generate three alternatively spliced mRNAs, each of which encodes a distinct protein isoform

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For immunoprecipitation, cell lysates were prepared as before and rotated 1 mg proteins with antibody at 4 C overnight, then immunoprecipitated by Protein A/G beads

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