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Mice were housed in specific pathogen-free conditions with a 12: 12 h light/dark cycle with a temperature of 24 C 0.5 C and a relative humidity of 40%-70%

An important consequence of this increase in glutathione synthesis was a significant decline in both oxidative stress and plasma markers of oxidant damage in the elderly to levels similar to those in the young control subjects

GPX1, glutathione peroxidase 1

Similar effects can be seen with the binding of Cd 2+ , Hg 2+ and Pb 2+

Inhibition of growth with nitisinone Nitisinone, 2-(2-nitro-4-trifluoromethylbenzoyl)-cyclohexane-1, 3 dione, a potent specific inhibitor of 4-HPPD was purchased from Swedish Orphan Biovitrum, Sweden