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Western blotting analysis and enzyme-linked immunosorbent assay (ELISA) assay Whole liver lysates were prepared by sonication in HEPES lysis buffer (50 mM HEPES pH 7.5, 150 mM NaCl, 1 mM EDTA, 2.5 mM EGTA, 0.1% Tween 20, 10% glycerol, 0.1 mM Na orthovanadate, 1 mM NaF, 10 mM -glycerophosphate) containing protease inhibitor cocktail (EDTA-free Complete Mini, Roche, Meylan, France)

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Exercise is another great supporter of glutathione as it makes the antioxidants job easier by reducing oxidative stress

Upon its efflux from cells, oxidised glutathione is exposed to the sequential action of enzymes located on the external face of the cell membrane: -glutamyl transpeptidase (GGT) generates glutamate 94 and the cysteinylglycine dipeptide, which is degraded by dipeptidases such as aminopeptidase N (APN), thereby releasing cysteine and glycine

The lipid peroxides formed may be chemotactic for the neutrophils causing increased inflammation, which further drives oxidant-mediated injury in the liver